Identification of candidate antigens from adult stages of Toxocara canis for the serodiagnosis of human toxocariasis
Mem. Inst. Oswaldo Cruz
;
106(2): 200-206, Mar. 2011. ilus, graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-583946
ABSTRACT
In the present work, we identified adult Toxocara canis antigens through sodium dodecyl sulfate-polyacrylamide gel electrophoresis for potential use in human toxocariasis immunodiagnosis. The sensitivity and specificity of several semi-purified antigens, as well as their cross-reactivity with other parasitic infections, were assessed by IgM and IgG-enzime linked immunosorbent assay. Whilst we found that the crude extract of the parasite presented limited sensitivity, specificity and high cross-reactivity against other parasites, we identified 42, 58, 68 and 97-kDa semi-purified antigens as the most promising candidates for immunodiagnosis. Moreover, the 58 and 68-kDa antigens presented the lowest IgM cross-reactivity. When tested as a combination, a mixture of the 58 and 68-kDa antigens presented 100 percent sensitivity and specificity, as well as minor cross-reactivity. Although the combination of the 42, 58, 68 and 97-kDa antigens presented 100 percent sensitivity at a dilution of 1:40, the low specificity and high cross-reactivity observed suggested a limited use for diagnostic purposes. Our data suggested that the 58 and 68-kDa antigens might be most suitable for the immunodiagnosis of human toxocariasis.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Imunoglobulina G
/
Imunoglobulina M
/
Anticorpos Anti-Helmínticos
/
Toxocaríase
/
Toxocara canis
/
Antígenos de Helmintos
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2011
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Hospital Santa Casa de Belo Horizonte/BR
/
Universidade Federal de Minas Gerais/BR
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