Isolation and multiplex genotyping of polymorphic microsatellite DNA markers in the snakehead murrel, Channa striata

Jamsari, Amirul Firdaus Jamaluddin; Min-Pau, Tan; Siti-Azizah, Mohd Nor

Jamsari, Amirul Firdaus Jamaluddin; Min-Pau, Tan; Siti-Azizah, Mohd Nor.

Jamsari, Amirul Firdaus Jamaluddin; Universiti Sains Malaysia. School of Biological Sciences. Malaysia. MY
Min-Pau, Tan; Universiti Sains Malaysia. School of Biological Sciences. Malaysia. MY
Siti-Azizah, Mohd Nor; Universiti Sains Malaysia. School of Biological Sciences. Malaysia. MY

Genet. mol. biol ; 34(2): 345-347, 2011.

Artigo em Inglês | LILACS | ID: lil-587754

ABSTRACT

ABSTRACT

Seven polymorphic microsatellite loci were isolated and characterized for the snakehead murrel, Channa striata (Channidae), a valuable tropical freshwater fish species. Among 25 specimens collected from Kedah state in Malaysia, the number of alleles per locus ranged from 2 to 7. Observed and expected heterozygosities ranged from 0.120 to 0.880 and 0.117 to 0.698, respectively. A single locus (CS1-C07) was significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. These novel markers would be useful for population genetic studies of the C. striata.

Channa striata; Microsatellite; Multiplex PCR; Population genetics

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Texto completo: DisponíveL Índice: LILACS (Américas) Idioma: Inglês Revista: Genet. mol. biol Assunto da revista: Genética Ano de publicação: 2011 Tipo de documento: Artigo / Documento de projeto País de afiliação: Malásia Instituição/País de afiliação: Universiti Sains Malaysia/MY

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