Apoptosis and survivability of human dental pulp cells under exposure to Bis-GMA
J. appl. oral sci
;
19(3): 218-222, May-June 2011. graf
Artigo
em Inglês
| LILACS
| ID: lil-588125
ABSTRACT
OBJECTIVE:
In the present study, we examined whether 2, 2-bis [4-(2-hydroxy-3-methacryloxypropoxy) phenyl] propane (Bis-GMA) has effects on LSC2 cells, human dental pulp cell line. MATERIAL ANDMETHODS:
The viability, cell cycle, and morphology of LSC2 cells were analyzed after exposure to several different concentrations of Bis-GMA. The recovery of viability of Bis-GMA exposed cells was also analyzed in the condition without Bis-GMA. Further, penetration of Bis-GMA to dentin disc was examined using isocratic high-performance liquid chromatography.RESULTS:
There was a concentration-dependent decrease in cell proliferation and an increase in cell number in the sub-G1 population after exposure to Bis-GMA. Furthermore, the cells showed typical characteristics of apoptotic cells after the exposure to high concentration of Bis-GMA. In contrast, cells exposed to lower concentrations of Bis-GMA recovered their viability after being cultured without Bis-GMA. We also found that Bis-GMA is capable of penetrating 1-mm-thick dentin discs, though the penetrated concentration was lower than that showing cytotoxicity.CONCLUSION:
These results suggest that Bis-GMA has cytotoxic effects, though dental pulp exposed to lower concentrations is able to recover their viability when Bis-GMA is removed.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Técnicas In Vitro
/
Bis-Fenol A-Glicidil Metacrilato
/
Apoptose
/
Polpa Dentária
Limite:
Humanos
Idioma:
Inglês
Revista:
J. appl. oral sci
Assunto da revista:
Odontologia
Ano de publicação:
2011
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Japão
/
Taiwan
Instituição/País de afiliação:
Kagoshima University Graduate School of Medical and Dental Sciences/JP
/
Kaohsiung Medical University Hospital/TW
/
Kyushu Dental College/JP
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