Detection of single nucleotide polymorphisms in the conserved ESTs regions of Gossypium arboreum
Electron. j. biotechnol
;
13(5): 3-4, Sept. 2010. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-591885
ABSTRACT
Exploring genetic variation in Gossypium arboreum L. germplasm is useful as it contains many important genes conferring resistance to different stresses. In limited earlier studies, low level of genetic diversity was found by using conventional DNA marker systems which may impede future genome mapping studies. In the present investigation, we explored the extent of Single Nucleotide Polymorphisms (SNP) among 30 conserved regions of Expressed Sequence Tags (EST) of low copy genes between two genotypes of G. arboreum. A total of 27 SNPs including 21 substitutions and 6 Insertions and deletions (Indels) in 7804 bp were found between these genotypes with a frequency of one SNP per 371 bp and one Indel after every 1300 bp. Out of these SNPs, 52 percent were transitions, whilst 48 percent SNPs were transversion. In conclusion, SNPs are expedient markers that can explore polymorphism in highly conserved sequences where other markers are not effective.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Gossypium
/
Polimorfismo de Nucleotídeo Único
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
Paquistão
Instituição/País de afiliação:
National Institute for Biotechnology and Genetic Engineering/PK
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