Characterization of novel genic SSR markers in Linum usitatissimum (L. ) and their transferability across eleven Linum species
Electron. j. biotechnol
;
14(2): 4-4, Mar. 2011. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-591934
ABSTRACT
Little is known about the evolutionary relationships among Linum species, basically because of the lack of transferable molecular markers. Currently, expressed sequence tags available in public databases provide an opportunity for the rapid and inexpensive development of simple sequence repeat (SSR) markers in wild flax species. In this regard, fifty expressed sequence tag-derived microsatellite markers (EST-SSRs) were evaluated for polymorphism and transferability in 50 Linum usitatissimum cultivars/accessions and 11 Linum species. Among them 23 EST-SSRs were polymorphic in L. usitatissimum, while 2-4 alleles were detected (average 2.26 per locus). The polymorphism information content value ranged from 0.08 to 0.55 (average 0.38). Forty one genic markers (95.3 percent) produced strong amplicons in at least two of the 11 Linum species. The percentage of cross amplification ranged from 34.1 percent to 92.7 percent in L. tauricum and L. bienne, respectively. Moreover, the rate of transferability was associated positively with the botanical section. Our results suggest that the high degree of EST-SSRs transferability to Linum species can be a useful enhancement of the current database of SSR markers for future genetic and evolutionary studies.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Repetições de Microssatélites
/
Linho
/
Etiquetas de Sequências Expressas
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2011
Tipo de documento:
Artigo
País de afiliação:
Chile
Instituição/País de afiliação:
INIA-Carillanca/CL
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