Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells
Biocell
; 33(1): 39-47, Apr. 2009. ilus
Article
em En
| LILACS
| ID: lil-595028
Biblioteca responsável:
AR40.1
ABSTRACT
Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/A1 cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/A1 cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cel ls, which possess morphological and functional characteristics of odontoblast-like cells expressing dentin sialophosphoprotein in vivo. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells in vivo.
Texto completo:
1
Índice:
LILACS
Assunto principal:
Sialoglicoproteínas
/
Regeneração Óssea
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Colágeno Tipo I
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Odontoblastos
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biocell
Assunto da revista:
Clulas
Ano de publicação:
2009
Tipo de documento:
Article