NFAT regulates CSF-1 gene transcription triggered by L-selectin crosslinking
Biocell
;
34(2): 57-63, Aug. 2010. graf
Artigo
em Inglês
| LILACS
| ID: lil-595039
ABSTRACT
L-selectin is a member of the selectin family that play an important role both in mediating the initial capture and subsequent rolling of leukocytes along the endothelial cells. Furthermore, L-selectin can function as a signal molecule. In our previous studies, we reported that L-selectin ligation could regulate CSF-1 (colony-stimulating factor-1) gene transcription, in which AP-1 acts as a crucial transcriptional factor. Here we investigated the function of the NFAT in the CSF-1 gene transcriptional events. We found that overexpression of WT NFAT induce CSF-1 gene transcription greatly in the activated Jurkat cells. Furthermore, we found that NFAT can be recruited to the nucleus after L-selectin ligation, and the nuclear NFAT interacts with the CSF-1 promoter region to regulate CSF-1 gene transcription in the L-s electin ligation activated Jurkat cells. These results indicate that nuclear NFAT can activate CSF-1 gene transcription by connecting with the CSF-1 promoter in the signaling events induced by L-selectin ligation.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Linfócitos T
/
Selectina L
/
Células Jurkat
/
Fatores de Transcrição NFATC
/
Macrófagos
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Biocell
Assunto da revista:
Clulas
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
China University of Petroleum (East China)/CN
/
Northeast Normal University/CN
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