In vitro effects of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and cell cycle dynamics in the MCF-7 breast adenocarcinoma cell line
Biocell
;
34(2): 71-79, Aug. 2010. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-595041
ABSTRACT
In the search for new and improved anticancer therapies, researchers have identified several potentially useful compounds. One of these agents is 2-methoxyestradiol-bis-sulphamate (2ME-BM), a sulphamoylated derivative of 2-methoxyestradiol. The objective of this study was to evaluate 2ME-BM's in vitro efficacy as antiproliferative agent in the MCF-7 breast adenocarcinoma cell line. Light- and fluorescent microscopy showed decreased cell density, increased apoptotic characteristics and significant ultrastructural aberrations indicative of autophagic cell death after 24 hours of exposure at a concentration of 0.4 microM. In addition, mitotic indices revealed that 2ME-BM induces a G2M block. The latter was confirmed by flow cytometric analyses where increased sub-G1 and G2/M fractions, as well as an increase in cycli n B1 levels were observed. Further in vitro research into the mechanism of this potentially useful anticancer compound is thus warranted.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Ciclo Celular
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Estriol
Tipo de estudo:
Estudo prognóstico
Limite:
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Biocell
Assunto da revista:
Clulas
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
África do Sul
Instituição/País de afiliação:
University of Pretoria/ZA
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