Taxus globosa S. cell lines: initiation, selection and characterization in terms of growth, and of baccatin III and paclitaxel production
Biocell
;
34(1): 1-6, Apr. 2010. tab, ilus
Artigo
em Inglês
| LILACS
| ID: lil-595044
ABSTRACT
Of the initial six cell lines originating from explants of Taxus globosa, or Mexican yew (stem internode, leaves and meristematic tissue), three were selected for their microbial and oxidation resistance, two from leaves and the other from stem internode. A study of their behavior, both in terms of cell growth, and of baccatin III and paclitaxel production, was developed in suspension cultures with an initially standardized biomass (fresh weight 0.23 g/L) using modified Gamborg's B5 medium, and an elicitor (methyl jasmonate), on either the first or seventh day of culture, at several levels (0, 0.1, 1, 10, 100 microM). In most of the conditions used, the three cell lines showed growth associated baccatin III production. The cell line from stem internode was the highest producer of baccatin III using 1 microM elicit or, sampling at 10 days.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Paclitaxel
/
Taxus
/
Ciclopentanos
/
Proliferação de Células
/
Alcaloides
/
Acetatos
Idioma:
Inglês
Revista:
Biocell
Assunto da revista:
Clulas
Ano de publicação:
2010
Tipo de documento:
Artigo
País de afiliação:
México
Instituição/País de afiliação:
Instituto Tecnológico de Veracruz/MX
/
Instituto de Ecología/MX
/
Universidad Veracruzana/MX
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