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Cobalt chloride stimulates phosphoinositide 3-kinase/Akt signaling through the epidermal growth factor receptor in oral squamous cell carcinoma
Ryu, Mi Heon; Park, Jeong Hee; Park, Ji Eun; Chung, Jin; Lee, Chang Hun; Park, Hae Ryoun.
Afiliação
  • Ryu, Mi Heon; Pusan National University. School of Dentistry. Department of Oral Pathology. Yangsan. KR
  • Park, Jeong Hee; Pusan National University. School of Dentistry. Department of Oral Pathology. Yangsan. KR
  • Park, Ji Eun; Pusan National University. School of Dentistry. Department of Oral Pathology. Yangsan. KR
  • Chung, Jin; Pusan National University. School of Dentistry. Department of Oral Microbiology. Yangsan. KR
  • Lee, Chang Hun; Pusan National University. School of Dentistry. Department of Pathology. Yangsan. KR
  • Park, Hae Ryoun; Pusan National University. School of Dentistry. Department of Oral Pathology. Yangsan. KR
Biocell ; Biocell;34(1): 15-21, Apr. 2010. graf
Article em En | LILACS | ID: lil-595046
Biblioteca responsável: AR40.1
ABSTRACT
Tumor cells are often found under hypoxic conditions due to the rapid outgrowth of their vascular supply, and, in order to survive hypoxia, these cells induce numerous signaling factors. Akt is an important kinase in cell survival, and its activity is regulated by the upstream phosphoinositide 3-kinase (PI3K) and receptor tyrosine kinases (RTKs). In this study, we examined Akt activation and RTKs/PI3K/Akt signaling using the hypoxia-mimetic cobalt chloride in oral squamous carcinoma cells. Cobalt chloride increases Akt phosphorylation in both a dose- and time-dependent manner. Blocking the activation of the PI3K/Akt pathway using LY294002 abolished Akt activation in response to cobalt chloride, suggesting that Akt phosphorylation by cobalt chloride is dependent on PI3K. In addition, activation of the PI3K/Akt path way seems to rely on the epidermal growth factor receptor (EGFR), since the inhibition of EGFR attenuated cobalt chloride-induced Akt activation. The results in this study also demonstrate that cobalt chloride increases EGFR protein levels and induces oral squamous cell carcinoma cells to enter S phase.
Assuntos
Texto completo: 1 Índice: LILACS Assunto principal: DNA de Neoplasias / Neoplasias Bucais / Carcinoma de Células Escamosas / Hipóxia Celular / Cobalto / Proteínas Proto-Oncogênicas c-akt Limite: Humans Idioma: En Revista: Biocell / Biocell (En línea) / Biocell (Mendoza) Assunto da revista: C‚lulas Ano de publicação: 2010 Tipo de documento: Article
Texto completo: 1 Índice: LILACS Assunto principal: DNA de Neoplasias / Neoplasias Bucais / Carcinoma de Células Escamosas / Hipóxia Celular / Cobalto / Proteínas Proto-Oncogênicas c-akt Limite: Humans Idioma: En Revista: Biocell / Biocell (En línea) / Biocell (Mendoza) Assunto da revista: C‚lulas Ano de publicação: 2010 Tipo de documento: Article