Detección de ADN de parvovirus B19 en donantes de sangre de tres hospitales en Santiago, Chile / Parvovirus B19 among blood donors from three hospitals in Santiago, Chile
Rev. méd. Chile
;
139(2): 143-149, feb. 2011. ilus, tab
Artigo
em Espanhol
| LILACS
| ID: lil-595279
ABSTRACT
Background: Parvovirus B19 (B19) is associated with a wide range of diseases in humans, whose severity depends on the immunological and hematological status of the host. It is transmitted mainly through the airway but also by transfusions. Aim: To determine the B19 DNA carrier frequency in a population of volunteer blood donors from three hospitals blood banks in Santiago, Chile, and to determine the viral load in DNA positive cases. Material and Methods: A total of477 serum samples were analyzed. The screening of B19 DNA was carried out by nested polymerase chain reaction (PCR) directed to the non-structural region of the virus (NS1). The viral load in positives cases was quantified by NS1 Real Time PCR. Results: Parvovirus B19 was detected in four samples, rendering a frequency of 1:119. The viral loads ranged from less than 2000 to 5,626 x 10(5) genome equivalents/ml. Conclusions: Parvovirus B19 was present in four of 477 blood bank blood donors from three hospitals in Santiago.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Doadores de Sangue
/
DNA Viral
/
Infecções por Parvoviridae
Limite:
Adolescente
/
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
País/Região como assunto:
América do Sul
/
Chile
Idioma:
Espanhol
Revista:
Rev. méd. Chile
Assunto da revista:
Medicina
Ano de publicação:
2011
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Chile
Instituição/País de afiliação:
Universidad de Chile/CL
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