Recombinant expression and refolding of the c-type lysozyme from Spodoptera litura in E. coli
Electron. j. biotechnol
;
14(3): 6-6, May 2011. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-602983
ABSTRACT
The chicken-type lysozyme of the insect Spodoptera litura (SLLyz) is a polypeptide of 121 amino acids containing four disulfide bridges and 17 rare codons and participates in innate defense as an anti-bacterial enzyme. The recombinant S. litura lysozyme (rSLLyz) expressed as a C-terminal fusion protein with glutathione S-transferase (GST) in Rosetta(DE3) Singles. The protein was produced as an inclusion body which was solubilized in 8 M urea, renatured by on-column refolding, and purified by reversed-phase chromatography to 95 percent purity. The purified rSLLyz demonstrated antibacterial activity against B. megaterium confirmed by inhibition zone assay. The overexpression and refolding strategy described in this study will provide a reliable technique for maximizing production and purification of proteins expressed as inclusion bodies in E. coli.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Muramidase
/
Corpos de Inclusão
/
Spodoptera
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2011
Tipo de documento:
Artigo
País de afiliação:
Coréia do Sul
Instituição/País de afiliação:
Dankook University/KR
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