Bacterial biodiversity from an anaerobic up flow bioreactor with ANAMMOX activity inoculated with swine sludge
Braz. arch. biol. technol
;
54(5): 1035-1041, Sept.-Oct. 2011. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-604265
ABSTRACT
The present study aimed to describe the bacterial community present at an anaerobic up flow bioreactor with ANAMMOX activity, inoculated with the sludge from the anaerobic pond of a swine slurry treatment system. The description was based on the molecular DNA techniques using primers for amplification of complete 16S rRNA gene and also new primers to amplify smaller fragments from 16S rRNA. During the bioreactor operation time, the bacterial community changed significantly, increasing the nitrogen removal efficiency, reaching after 500 days a removal rate of 94 percent. The complete PCR amplification of 16S rRNA gene generated 17 clones, where three presented similarity with Candidatus Jettenia asiatica (97 percent), twelve with Janthinobacterium (99 percent) and two with uncultured clones. The PCR amplification of 436 base pairs had generated 12 clones, of which eight presented 96-100 percent similarity with Candidatus Anammoxoglobus propionicus, Planctomycete KSU-1 and one with Pseudomonas sp. (99 percent) and three with uncultured clones.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Idioma:
Inglês
Revista:
Braz. arch. biol. technol
Assunto da revista:
Biologia
Ano de publicação:
2011
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Japão
/
Estados Unidos
Instituição/País de afiliação:
Embrapa Suinos e Aves/BR
/
Sojo University/JP
/
United States Department of Agriculture/US
/
Universidade Federal de Santa Catarina/BR
/
Universidade Federal de Santa Maria/BR
/
University Kurokami/JP
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