Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids
Genet. mol. biol
;
34(4): 640-646, 2011. ilus
Artigo
em Inglês
| LILACS
| ID: lil-605922
ABSTRACT
Khat (Catha edulis Forsk.) is a flowering perennial shrub cultivated for its neurostimulant properties resulting mainly from the occurrence of (S)-cathinone in young leaves. The biosynthesis of (S)-cathinone and the related phenylpropylamino alkaloids (1S,2S)-cathine and (1R,2S)-norephedrine is not well characterized in plants. We prepared a cDNA library from young khat leaves and sequenced 4,896 random clones, generating an expressed sequence tag (EST) library of 3,293 unigenes. Putative functions were assigned to > 98 percent of the ESTs, providing a key resource for gene discovery. Candidates potentially involved at various stages of phenylpropylamino alkaloid biosynthesis from L-phenylalanine to (1S,2S)-cathine were identified.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Fenilpropanolamina
/
Catha
Idioma:
Inglês
Revista:
Genet. mol. biol
Assunto da revista:
Genética
Ano de publicação:
2011
Tipo de documento:
Artigo
País de afiliação:
Canadá
/
Israel
Instituição/País de afiliação:
Ben-Gurion University of The Negev/IL
/
Medicinal and Spice Crops, Newe Ya'ar Research Center/IL
/
University of Calgary/CA
/
University of Western Ontario/CA
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