Expression of TGF-β1 in the blood during fracture repair in an estrogen-deficient rat model
Clinics
;
66(12): 2113-2119, 2011. graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-609010
ABSTRACT
OBJECTIVES:
Previous studies have reported that osteoporosis due to estrogen deficiency influences fracture healing. Transforming growth factor (TGF-b) has been found to be involved in fracture healing via the regulation of the differentiation and activation of osteoblasts and osteoclasts. The current study aimed to determine the effects of estrogen on the expression of TGF-β1 during fracture healing in ovariectomized rats.METHODS:
Thirty female Sprague-Dawley rats weighing 200-250 g were assigned to (i) a sham-operated group that was given a normal saline; (ii) an ovariectomized control group that was given a normal saline; or (iii) an ovariectomized + estrogen (100 mg/kg/day) group that was treated with conjugated equine estrogen. The right femur of all rats was fractured, and a Kirschner wire was inserted six weeks post-ovariectomy. Treatment with estrogen was given for another six weeks post-fracture. At the end of the study, blood samples were taken, and the right femur was harvested and subjected to biomechanical strength testing.RESULTS:
The percentage change in the plasma TGF-β1 level before treatment was significantly lower in the ovariectomized control and estrogen groups when compared with the sham group (p<0.001). After six weeks of treatment, the percentage change in the plasma TGF-β1 level in the estrogen group was significantly higher compared with the level in the ovariectomized control group (p = 0.001). The mean ultimate force was significantly increased in the ovariectomized rats treated with estrogen when compared with the ovariectomized control group (p = 0.02).CONCLUSION:
These data suggest that treatment with conjugated equine estrogen enhanced the strength of the healed bone in estrogen-deficient rats by most likely inducing the expression of TGF-β1.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Osteoporose
/
Consolidação da Fratura
/
Estrogênios
/
Fator de Crescimento Transformador beta1
/
Fraturas do Fêmur
Tipo de estudo:
Estudo de etiologia
/
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Clinics
Assunto da revista:
Medicina
Ano de publicação:
2011
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Malásia
Instituição/País de afiliação:
Universiti Kebangsaan Malaysia Medical Center/MY
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