A direct method for cDNA cloning employing translation released mRNA
Braz. j. med. biol. res
;
20(3/4): 313-20, 1987. ilus
Artigo
em Inglês
| LILACS
| ID: lil-61007
RESUMO
1. A new cloning procedure is described for cDNA synthesis from mRNA released by in vitro translation of polysomes in a cell-free amino acid incorporating system. The usefulness of the method lies in the feasibility of employing nanogram amounts of mRNA. 2. Complementary DNA is synthesized derectly in the translation mixture simply by adjusting the concentration of some components and removing ribosomes by boiling and centrifugation. 3. As an example, we report here the construction and characterization of cDNA clone corresponding to chick alfa (1) procollagen starting from a collagen-synthesizing polysome fraction obtained from chick embryos
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Índice:
LILACS (Américas)
Assunto principal:
Polirribossomos
/
Técnicas In Vitro
/
DNA Recombinante
/
RNA Mensageiro
/
Clonagem Molecular
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1987
Tipo de documento:
Artigo
/
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