Construction of a gene library from Azospirillum brasilense and characterization of a recombinant containing the nif structural genes
Braz. j. med. biol. res
;
20(3/4): 321-30, 1987. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-61008
RESUMO
1.We have constructed a gene library, from Azospirillum brasilense using the vector EMBL4. 2. A recombinant containing the nif structural genes from A. brasilense was isolated and characterized. This recombinant contains a DNA insert of about 15 kilobases (KB) which gives rise to five fragments after cleavage with EcoRI. Only one of the DNA fragments (6.5 Kb) hybridized to the nifHDK genes of Klebsiella pneumoniae. 3 The organization of the nif genes in this DNA fragment was determined using different DNA segments containing the nifH, nifK or nifD genes of K. pneumoniae as probes
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Índice:
LILACS (Américas)
Assunto principal:
Spirillum
/
DNA Recombinante
/
Genes
/
Genes Bacterianos
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1987
Tipo de documento:
Artigo
/
Documento de projeto
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