Brewer's spent grain and corn steep liquor as alternative culture medium substrates for proteinase production by Streptomyces malaysiensis AMT-3
Braz. j. microbiol
;
42(4): 1384-1389, Oct.-Dec. 2011. ilus
Artigo
em Inglês
| LILACS
| ID: lil-614599
ABSTRACT
Brewer's spent grain and corn steep liquor or yeast extract were used as the sole organic forms for proteinase production by Streptomyces malaysiensis in submerged fermentation. The influence of the C and N concentrations, as well as the incubation periods, were assessed. Eight proteolytic bands were detected through gelatin-gel-electrophoresis in the various extracts obtained from the different media and after different incubation periods, with apparent molecular masses of 20, 35, 43, 50, 70, 100, 116 and 212 kDa. The results obtained suggest an opportunity for exploring this alternative strategy for proteinases production by actinomycetes, using BSG and CSL as economically feasible substrates.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Peptídeo Hidrolases
/
Saccharomyces cerevisiae
/
Streptomyces
/
Actinobacteria
/
Fermentação
Tipo de estudo:
Estudos de avaliação
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2011
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal do Recôncavo da Bahia/BR
/
Universidade Federal do Rio de Janeiro/BR
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