Prevalence of glutathione S-transferase gene deletions and their effect on sickle cell patients
Rev. bras. hematol. hemoter
;
34(2): 100-102, 2012. tab
Artigo
em Inglês
| LILACS
| ID: lil-624766
ABSTRACT
BACKGROUND:
Glutathione S-transferase gene deletions are known detoxification agents and cause oxidative damage. Due to the different pathophysiology of anemia in thalassemia and sickle cell disease, there are significant differences in the pathophysiology of iron overload and iron-related complications in these disorders.OBJECTIVE:
The aim of this study was to estimate the frequency of the GSTM1 and GSTT1 genotypes in sickle cell disease patients and their effect on iron status.METHODS:
Forty sickle cell anemia and sixty sickle ß-thalassemia patients and 100 controls were evaluated to determine the frequency of GST gene deletions. Complete blood counts were performed by an automated cell analyzer. Hemoglobin F, hemoglobin A, hemoglobin A2 and hemoglobin S were measured and diagnosis of patients was achieved by high performance liquid chromatography with DNA extraction by the phenol-chloroform method. The GST null genotype was determined using multiplex polymerase chain reaction and serum ferritin was measured using an ELISA kit. Statistical analysis was by EpiInfo and GraphPad statistics software.RESULTS:
An increased frequency of the GSTT1 null genotype (p-value = 0.05) was seen in the patients. The mean serum ferritin level was higher in patients with the GST genotypes than in controls; this was statistically significant for all genotypes except GSTM1, however the higher levels of serum ferritin were due to blood transfusions in patients.CONCLUSION:
GST deletions do not play a direct role in iron overload of sickle cell patients.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Hemoglobina Falciforme
/
Reação em Cadeia da Polimerase
/
Glutationa Transferase
/
Hemoglobinopatias
/
Anemia Falciforme
Tipo de estudo:
Estudo de prevalência
/
Fatores de risco
Limite:
Humanos
Idioma:
Inglês
Revista:
Rev. bras. hematol. hemoter
Assunto da revista:
Hematologia
Ano de publicação:
2012
Tipo de documento:
Artigo
País de afiliação:
Índia
Instituição/País de afiliação:
All India Institute of Medical Sciences/IN
/
Awadhesh Pratap Singh University Rewa/IN
/
Institute of Medical Sciences/IN
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