Effect of serum sample inactivation on the performance of latex agglutination test for paracoccidioidomycosis serodiagnosis
Mem. Inst. Oswaldo Cruz
;
107(4): 510-512, June 2012. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-626445
ABSTRACT
Paracoccidioidomycosis is diagnosed from the direct observation of the causative agent, but serology can facilitate and decrease the time required for diagnosis. The objective of this study was to determine the influence of serum sample inactivation on the performance of the latex agglutination test (LAT) for detecting antibodies against Paracoccidioides brasiliensis. The sensitivity of LAT from inactivated or non-inactivated samples was 73% and 83%, respectively and the LAT selectivity was 79% and 90%, respectively. The LAT evaluated here was no more specific than the double-immunodiffusion assay. We suggest the investigation of other methods for improving the LAT, such as the use of deglycosylated antigen.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Paracoccidioides
/
Paracoccidioidomicose
/
Manejo de Espécimes
/
Testes de Fixação do Látex
/
Anticorpos Antifúngicos
Tipo de estudo:
Estudo diagnóstico
Limite:
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2012
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal do Pará/BR
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