Inmunovaloración de anticuerpos IgM en muestras sospechosas de sarampión clínicamente

RESUMEN

Se realiza el estudio de 60 sueros pareados sospechosos de Sarampión clínicamente llegados al Laboratorio Diagnóstico del Instituto de Medicina Tropical "Pedro Kourí" entre enero y mayo de 1996, procedentes de la vigilancia seroepidemiológica de la vacuna triple viral (sarampion, rubéola y parotiditis), a los cuales se les realizó la detección de anticuerpos hemaglutinantes a sarampión y rubéola, así como de IgM, con el estuche diagnóstico Clark Laboratories INC Measles IgM ELISA. Los casos positivos se confirmaron por las técnicas de inmunofluorescencia indirecta-IgM y neutralización. Se obtuvieron por inhibición de la hemaglutinación, 3 casos positivos a sarampión y rubéola, los cuales negativos a IgM de sarampión y a los que se les determinaron anticuerpos a Epstein Barr, Citomegalovirus y al virus herpes simple mediante inmunifluorescencia indirecta (IFI) la capacidad de estos virus de inducir respuesta policlonal. Por medio del ELISA IgM Clark se detectaron 6 casos positivos los cuales resultaron negativos por IFI.

ABSTRACT

60 matched sera clinically syspicious of measles that were received at the Diagnostic Laboratory of the "Pedro Kourí" Tropical Medicine Institute between January and May, 1996, coming from the seroepidemiological surveillance of the MPR vaccine were studied. The detection of measles and rubella hemagglutinant antibodies, as well as of IgM, was carried out with the Clark Laboratories INC. Measles IgM ELISA diagnostic kit. The positive cases were confirmed by the IgM-indirect immunofluorescence and neutralization. 3 positive cases to measles and rubella, which were negative to measles IgM, were obtained by hemagglutination inhibition. Antibodies against Epstein Barr, cytomegalovirus and herpes simplex virus were also determined by indirect immunofluorescence (IIF) due to the capacity of these viruses to induces polyclonal responses. 6 positive cases, which were negative by IIF, were detected by means of the above mentioned diagnostic kit.