Bone marrow mesenchymal cell adhesion to polished and nitrided titanium surfaces

ABSTRACT

Aim: To evaluate the adhesion of mouse bone marrow mesenchymal cells (MBMMC) on differenttitanium surfaces. Methods: Grade II titanium discs (ASTM F86) received two different surfacetreatments polished (S1) and cathodic cage plasma nitriding (S2). MBMMC were cultured ontitanium discs in 24-well cell culture plates, at a density of 1 x 104 cells per well. After 24 h, theadhesion was evaluated using a hemocytometer. Results: The mean adhesion was greater onS2, though without statistically significant difference from S2 (p>0.05). Conclusions: It wasdemonstrated that titanium surface treatment with ionic nitriding in a cathodic cage is biocompatiblesince it preserved the integrity of the cultivated MBMMC for a period of 24 h, allowing theiradhesion.