Expression of the antibody 14D9 in Nicotiana tabacum hairy roots
Electron. j. biotechnol
;
8(2): 51-57, Aug. 2005. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-640475
ABSTRACT
Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 µg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 µg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 µg and 14 µg respectively.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Idioma:
Inglês
Revista:
Electron. j. biotechnol
Assunto da revista:
Biotecnologia
Ano de publicação:
2005
Tipo de documento:
Artigo
País de afiliação:
Argentina
Instituição/País de afiliação:
CIDCA/AR
/
Universidad de Buenos Aires/AR
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