Synergistic haemolytic activity and its correlation to phospholipase D productivity by Corynebacteruim pseudotuberculosis Egyptian isolates from sheep and buffaloes
Braz. j. microbiol
;
43(2): 552-559, Apr.-June 2012. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-644470
ABSTRACT
Fourteen isolates of Corynebacteruim pseudotuberculosis of them 7 were isolated from sheep with Caseous Lymphadenitis "biotype 1" and 7 isolated from buffaloes with Oedematous Skin Disease "biotype 2". All isolates were identified by standard microbiological techniques and by polymerase chain reaction targeting, 16S rRNA and phospholipase D genes. Synergistic haemolytic titers of all isolates were assayed by plate technique. The presences of phospholipase D gene in supernatants of all isolates were performed by sodium dodecyl sulfate polyacrylamide gel electrophoresis immunoblot technique by using hyperimmune serum raised in rabbit immunized with recombinant phospholipase D gene antigen. The concentration of phospholipase D gene was assayed by scanning the bound phospholipase D gene with specific antibodies that appeared at 31.5 kDa. Results presented that there is no correlation between titer of Synergistic haemolytic activity and the actual phospholipase D genes concentration in culture supernatants. Also results presented that Synergistic haemolytic activity and phospholipase D genes produced by biotype 2 (buffalo isolates) was generally higher than those by biotype 1(sheep isolates).
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Fosfolipase D
/
Técnicas In Vitro
/
RNA
/
Regulação da Expressão Gênica
/
Corynebacterium pseudotuberculosis
/
Infecções por Corynebacterium
/
Linfadenite
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2012
Tipo de documento:
Artigo
País de afiliação:
Egito
/
Arábia Saudita
Instituição/País de afiliação:
Cairo University/EG
/
King Saud University/SA
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