Expression of phenazine biosynthetic genes during the arbuscular mycorrhizal symbiosis of Glomus intraradices
Braz. j. microbiol
; 43(2): 716-738, Apr.-June 2012. ilus, tab
Article
em En
| LILACS
| ID: lil-644490
Biblioteca responsável:
BR32.1
ABSTRACT
To explore the molecular mechanisms that prevail during the establishment of the arbuscular mycorrhiza symbiosis involving the genus Glomus, we transcriptionally analysed spores of Glomus intraradices BE3 during early hyphal growth. Among 458 transcripts initially identified as being expressed at presymbiotic stages, 20% of sequences had homology to previously characterized eukaryotic genes, 30% were homologous to fungal coding sequences, and 9% showed homology to previously characterized bacterial genes. Among them, GintPbr1a encodes a homolog to Phenazine Biosynthesis Regulator (Pbr) of Burkholderia cenocepacia, an pleiotropic regulatory protein that activates phenazine production through transcriptional activation of the protein D isochorismatase biosynthetic enzyme phzD (Ramos et al., 2010). Whereas GintPbr1a is expressed during the presymbiotic phase, the G. intraradices BE3 homolog of phzD (BGintphzD) is transcriptionally active at the time of the establishment of the arbuscular mycorrhizal symbiosis. DNA from isolated bacterial cultures found in spores of G. intraradices BE3 confirmed that both BGintPbr1a and BGintphzD are present in the genome of its potential endosymbionts. Taken together, our results indicate that spores of G. intraradices BE3 express bacterial phenazine biosynthetic genes at the onset of the fungal-plant symbiotic interaction.
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LILACS
Assunto principal:
Fenazinas
/
Simbiose
/
Técnicas In Vitro
/
Sequência de Bases
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Reação em Cadeia da Polimerase
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Hifas
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Micorrizas
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Enzimas
Tipo de estudo:
Prevalence_studies
/
Risk_factors_studies
Idioma:
En
Revista:
Braz. j. microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2012
Tipo de documento:
Article