Rhinovirus detection using different PCR-based strategies
Braz. j. microbiol
;
43(2): 739-743, Apr.-June 2012. tab
Artigo
em Inglês
| LILACS
| ID: lil-644491
ABSTRACT
Human rhinoviruses (HRVs) are the major cause of the common cold. HRVs were recently reclassified into the Enterovirus genus (HEV) in the Picornaviridae family. HRVs and other members of the HEV genus share many common features, including sense RNA genomes and partial nucleotide sequence identity. The aim of this study was to evaluate different HRV detection strategies. Samples from adults with acute respiratory infection (n = 291) who were treated in Sao Paulo Hospital (2001-2003) were tested using three assays. The first assay detected picornaviruses by RT-PCR and hybridization, the second detected rhinoviruses using RT-PCR/sequencing, and the third differentiated HRV from HEV using duplex semi-nested-RT-PCR. Analysis of the results obtained from the first two strategies revealed 83% concordance. Discordant samples were then evaluated by the third protocol, and 82% were negative. The picornavirus detection protocol was more sensitive but less specific than the rhinovirus detection protocols. The semi-nested protocol utilized in the present study was less sensitive and was not useful in differentiating HRV from HEV. Sequencing assays examining different genes would address the best strategy of confirming rhinovirus and enterovirus infections.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Picornaviridae
/
Infecções Respiratórias
/
Rhinovirus
/
Técnicas In Vitro
/
Sequência de Bases
/
Reação em Cadeia da Polimerase
/
Genoma Viral
/
Resfriado Comum
/
Infecções por Picornaviridae
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2012
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal de Sao Paulo/BR
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