Virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with freshwater ornamental fish culture systems: a possible threat to public health
Braz. j. microbiol
;
43(2): 754-765, Apr.-June 2012. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-644493
ABSTRACT
Aeromonas spp. are ubiquitous aquatic organisms, associated with multitude of diseases in several species of animals, including fishes and humans. In the present study, water samples from two ornamental fish culture systems were analyzed for the presence of Aeromonas. Nutrient agar was used for Aeromonas isolation, and colonies (60 No) were identified through biochemical characterization. Seven clusters could be generated based on phenotypic characters, analyzed by the programme NTSYSpc, Version 2.02i, and identified as Aeromonas caviae (33.3%), A. jandaei (38.3%) and A. veronii biovar sobria (28.3%). The strains isolated produced highly active hydrolytic enzymes, haemolytic activity and slime formation in varying proportions. The isolates were also tested for the enterotoxin genes (act, alt and ast), haemolytic toxins (hlyA and aerA), involved in type 3 secretion system (TTSS ascV, aexT, aopP, aopO, ascF-ascG, and aopH), and glycerophospholipid-cholesterol acyltransferase (gcat). All isolates were found to be associated with at least one virulent gene. Moreover, they were resistant to frequently used antibiotics for human infections. The study demonstrates the pathogenic potential of Aeromonas, associated with ornamental fish culture systems suggesting the emerging threat to public health.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Microbiologia da Água
/
Técnicas In Vitro
/
Resistência Microbiana a Medicamentos
/
Aciltransferases
/
Fauna Aquática
/
Reação em Cadeia da Polimerase
/
Infecções por Bactérias Gram-Negativas
/
Aeromonas
/
Suscetibilidade a Doenças
/
Enterotoxinas
Tipo de estudo:
Fatores de risco
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2012
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Índia
Instituição/País de afiliação:
Cochin University of Science and Technology/IN
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