Upregulation of matrix synthesis in chondrocyte-seeded agarose following sustained bi-axial cyclic loading
Clinics
;
67(8): 939-944, Aug. 2012. ilus, graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-647799
ABSTRACT
OBJECTIVES: The promotion of extracellular matrix synthesis by chondrocytes is a requisite part of an effective cartilage tissue engineering strategy. The aim of this in vitro study was to determine the effect of bi-axial cyclic mechanical loading on cell proliferation and the synthesis of glycosaminoglycans by chondrocytes in threedimensional cultures. METHOD: A strain comprising 10% direct compression and 1% compressive shear was applied to bovine chondrocytes seeded in an agarose gel during two 12-hour conditioning periods separated by a 12-hour resting period. RESULTS: The bi-axial-loaded chondrocytes demonstrated a significant increase in glycosaminoglycan synthesis compared with samples exposed to uni-axial or no loading over the same period (p<0.05). The use of a free-swelling recovery period prior to the loading regime resulted in additional glycosaminoglycan production and a significant increase in DNA content (p<0.05), indicating cell proliferation. CONCLUSIONS: These results demonstrate that the use of a bi-axial loading regime results in increased matrix production compared with uni-axial loading.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Regulação para Cima
/
Condrócitos
/
Matriz Extracelular
/
Glicosaminoglicanos
Limite:
Animais
Idioma:
Inglês
Revista:
Clinics
Assunto da revista:
Medicina
Ano de publicação:
2012
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Malásia
Instituição/País de afiliação:
University of Malaya/MY
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