Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits
Int. braz. j. urol
;
38(4): 536-543, July-Aug. 2012. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-649448
ABSTRACT
OBJECTIVE:
To investigate the VEGF expression and collagen deposition using a latex biomembrane as bladder replacement in rabbits. MATERIALS ANDMETHODS:
After partial cystectomy, a patch of a non-vulcanized latex biomembrane (2 x 2 cm) was sewn to the bladder of rabbits with 5/0 monofilament polydioxanone sulfate sutures in a watertight manner. Groups of 5 animals were killed at 15, 45 and 90 days after surgery and the bladder was removed. Sections of 5µm were cut and stained with picrosirius-red in order to estimate the amount of extracellular matrix in the graft. To confirm the presence of VEGF in tissues, protein expression was determined by immunohistochemistry.RESULTS:
No death, urinary leakage or graft extrusion occurred in any group. All bladders showed a spherical shape. A progressive reduction in the amount of collagen occurred in the graft area and was negatively and linearly correlated with time (p < 0.001). VEGF expression was higher in grafted areas when compared to controls at 15 and 45 days after surgery and decreased with time (p < 0.001).CONCLUSION:
The latex biomembrane as a matrix for partial bladder replacement in rabbits promotes temporary collagen deposition and stimulates the angiogenic process.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Materiais Biocompatíveis
/
Bexiga Urinária
/
Colágeno
/
Fator A de Crescimento do Endotélio Vascular
/
Látex
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Int. braz. j. urol
Assunto da revista:
Urologia
Ano de publicação:
2012
Tipo de documento:
Artigo
País de afiliação:
Brasil
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