An alternative method to isolate protease and phospholipase A2 toxins from snake venoms based on partitioning of aqueous two-phase systems
J. venom. anim. toxins incl. trop. dis
;
18(3): 306-316, 2012. ilus, graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-649478
ABSTRACT
Snake venoms are rich sources of active proteins that have been employed in the diagnosis and treatment of health disorders and antivenom therapy. Developing countries demand fast economical downstream processes for the purification of this biomolecule type without requiring sophisticated equipment. We developed an alternative, simple and easy to scale-up method, able to purify simultaneously protease and phospholipase A2 toxins from Bothrops alternatus venom. It comprises a multiple-step partition procedure with polyethylene-glycol/phosphate aqueous two-phase systems followed by a gel filtration chromatographic step. Two single bands in SDS-polyacrylamide gel electrophoresis and increased proteolytic and phospholipase A2 specific activities evidence the homogeneity of the isolated proteins.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Peptídeo Hidrolases
/
Venenos de Crotalídeos
Limite:
Animais
Idioma:
Inglês
Revista:
J. venom. anim. toxins incl. trop. dis
Assunto da revista:
Toxicologia
Ano de publicação:
2012
Tipo de documento:
Artigo
País de afiliação:
Argentina
Instituição/País de afiliação:
National University of Rosario/AR
/
Northeast National University/AR
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