Anti-galactosyl antibodies in chagasic patients: possible biological significance
Braz. j. med. biol. res
; 21(6): 1275-86, 1988. ilus, tab
Article
em En
| LILACS
| ID: lil-65029
Biblioteca responsável:
BR26.1
ABSTRACT
Antibodies from Chagasic patients (Ch), bound to a column of immobilized purified glycoprotein 25-kDa antigen of Trypanosoma cruzi epimastigotes (gp25), were partially lytic (70% lysis at 50 microng/ml) to cultured metacyclic trypomastigotes (Y strain) in a complement-mediated reaction (alternative pathway). Antibodies (Ch) eluted with galactose from a melibiose-Agarose column reacted in ELISA titration plates with gp25, whole cells of metacyclic trypomastigotes (Y strain), and less intensely with aqueous extracts of either T. cruzi (CL- 14 strain) or Herpetomonas muscarum. Agglutination of rabbit erythrocytes by anti-Gal antibodies from Chagasic patients or normal human sera (NHS) was inhibited by galactose and alfa- but not ß- anomeric galactosides. Anti-Gal antibodies (Ch or NHS) were lytic to metacyclic trypomastigotes in complement-mediated reactions. Agglutination of rabbit red cells by a serum sample from a T. cruzi-infected Rhesus monkey was inhibited by laminin. This serum was also lytic to metacyclic forms of T. cruzi (Y strain) involving the alternative complement pathway (70% lysis at 120 final dilution). These results open the possibility of using anti-Gal antibodies for protection against infection by virulent T. cruzi
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Índice:
LILACS
Assunto principal:
Anticorpos Antiprotozoários
/
Glicoproteínas
/
Doença de Chagas
/
Galactose
/
Antígenos de Protozoários
Limite:
Humans
Idioma:
En
Revista:
Braz. j. med. biol. res
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
1988
Tipo de documento:
Article
/
Congress and conference