Diversity of leaf endophytic fungi in mangrove plants of Northeast Brazil

ABSTRACT

With the aim of increasing the knowledge about endophytic fungi, a group of microorganisms with high biotechnological potential and a valuable source of useful metabolites, a survey in leaves of mangrove plants (Avicennia schaueriana, Laguncularia racemosa, and Rhizophora mangle) was performed at the Itamaracá Island, PE, Brazil. Leaves were collected, during two seasons, dry and rainy, superficially sterilized and fragments maintained in Petri dishes with Potato dextrose agar (PDA) at 28º ± 2º C until isolation of the fungi. Fourty taxa were isolated 25 species representing 19 genera and 15 morphotypes determined as Mycelia sterilia. Leaves of L. racemosa hosted the highest number of colony forming units (CFU) and taxa. Guignardia sp. and Colletotrichum gloeosporioides were the most frequently isolated, while Glomerella cingulata was the only species found in association with the three host plants. The proportional importance of each fungus differed among hosts. The similarity of fungi species between the two seasons reached only 4.2%, and that between the hosts was also low, with the maximum (A. schaueriana x L. racemosa) reaching 24.2%. Sphaerosporium, as well as Chloridium virescens var. virescens, Microsphaeropsis arundinis, Penicillium pinophilum, Periconia cambrensis, Phoma herbarum, P. diachenii, P. obscurans, Sordaria prolifica and Torula elisii are reported for the first time as endophytic in tropical regions.