'Synthetic lipase' production from a newly isolated Sporidiobolus pararoseus strain by submerged fermentation
Braz. j. microbiol
;
43(4): 1490-1498, Oct.-Dec. 2012. ilus, graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-665836
ABSTRACT
The lipase produced by a newly isolate Sporidiobolus pararoseus strain has potential catalysis ability for esterification reactions. In order to improve its synthetic activity, this work aimed at optimizing 'synthetic lipase' production by submerged fermentation of a conventional media based on peptone, yeast extract, NaCl and olive oil using experimental design technique. According to the results obtained in the first experimental design (2(4-1)), yeast extract and NaCl concentrations were tested to further optimization by response surface methodology. The maximum 'synthetic lipase' activity obtained was 26.9 U/mL in the optimized media (5.0, 6.8, 7.0 and 1.0% (wt/v) of peptone, yeast extract, NaCl and olive oil, respectively), representing a 6.36-fold increase compared to the initial medium. The time course of 'synthetic lipase' production in the optimized condition was evaluated in terms of synthetic activity, protease activity, biomass and total carbon and the maximum synthetic activity was observed during the stationary phase of growth.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Leveduras
/
Blastomyces
/
Sequência de Bases
/
Fungos Mitospóricos
/
Fermentação
/
Lipase
Tipo de estudo:
Estudos de avaliação
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2012
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal da Fronteira do Sul/BR
/
Universidade Federal de Santa Catarina/BR
/
Universidade Regional Integrada do Alto Uruguai e das Missões/BR
/
Universidade de São Paulo/BR
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