Optimization of flask culture medium and conditions for hyaluronic acid production by a streptococcus equisimilis mutant nc2168
Braz. j. microbiol
;
43(4): 1553-1561, Oct.-Dec. 2012. ilus, graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-665843
ABSTRACT
A mutant designated NC2168, which was selected from wild-type Streptococcus equisimilis CVCC55116by ultraviolet ray combined with60Co-γ ray treatment and does not produce streptolysin, was employed to produce hyaluronic acid (HA). In order to increase the output of HA in a flask, the culture medium and conditions for NC2168 were optimized in this study. The influence of culture medium ingredients including carbon sources, nitrogen sources and metal ions on HA production was evaluated using factional factorial design. The mathematical model, which represented the effect of each medium component and their interaction on the yield of HA, was established by the quadratic rotary combination design and response surface method. The model estimated that, a maximal yield of HA could be obtained when the concentrations of yeast extract, peptone, glucose, and MgSO4 were set at 3 g/100 mL, 2 g/100 mL, 0.5 g/100 mL and 0.15 g/100 mL, respectively. Compared with the values obtained by other runs in the experimental design, the optimized medium resulted in a remarkable increase in the output of HA and the maximum of the predicted HA production was 174.76 mg/L. The model developed was accurate and reliable for predicting the production of HA by NC2168.Cultivation conditions were optimized by an orthogonal experimental design and the optimal conditions were as follows temperature 33ºC, pH 7.8, agitation speed 200 rpm, medium volume 20 mL.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Infecções Estreptocócicas
/
Estreptolisinas
/
Streptococcus equi
/
Meios de Cultura
/
Ácido Hialurônico
Tipo de estudo:
Estudos de avaliação
/
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2012
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
China
Instituição/País de afiliação:
Academy of Agricultural Sciences/CN
/
Academy of Agriculture and Forestry Sciences/CN
/
Beijing Institute of landscape architecture/CN
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