Functional activity of neutrophils and systemic inflammatory response of Downs syndrome patients with periodontal disease
Braz. j. oral sci
;
11(3): 422-427, jul.-set. 2012. ilus, tab
Artigo
em Inglês
| LILACS, BBO
| ID: lil-667685
ABSTRACT
Periodontal disease (PD) is characterized as an inflammatory process that compromises the support and protection of the periodontium. Patients with Downs syndrome (DS) are prone to develop PD. Neutrophils (NE) are the first line of defense against infection and their absence sets the stage for disease. Aim:
To compare the activity and function of NE in the peripheral blood from DS patients with and without PD, assisted at the Center for Dental Assistance to Patients with Special Needs affiliated with the School of Dentistry of Araçatuba, Brazil.Methods:
Purified NE were collected from peripheral blood of 22 DS patients. NE were used to detect the 5-lypoxigenase (5-LO) expression by RT-PCR. Plasma from peripheral blood was collected to measure tumor necrosis factor-a (TNF-a) and interleukin-8 (IL-8) by ELISA and nitrite (NO3) using a Griess assay.Results:
Data analysis demonstrated that DS patients with PD present high levels of TNF-a and IL-8 when compared with DS patients without PD. However, there was no statistically significant difference in the levels of NO3 production between the groups. The levels of the inflammatory mediator 5-LO expression increased in DS patients with PD.Conclusions:
According with these results, it was concluded that TNF-a and IL-8 are produced by DS patients with PD. Furthermore, DS patients with PD presented high levels of 5-LO expression, suggesting the presence of leukotriene B4 (LTB4) in PD, thus demonstrating that the changes in NE function due to the elevation of inflammatory mediators contribute to PD.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Periodonto
/
Síndrome de Down
/
Fatores de Necrose Tumoral
/
Neutrófilos
Idioma:
Inglês
Revista:
Braz. j. oral sci
Assunto da revista:
Odontologia
Ano de publicação:
2012
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estadual Paulista/BR
Similares
MEDLINE
...
LILACS
LIS