Wound healing potential of aqueous crude extract of Stevia rebaudiana in mice

ABSTRACT

The present study was carried out to evaluate the wound healing potential of crude aqueous extract of Stevia rebaudiana (Bertoni) Bertoni, Asteraceae, in experimental animals. All experiments were conducted following standard procedures. The crude extract was administered topically in graded doses of 150, 250 and 500 mg/kg b.w. was used for evaluating the wound healing potential in excision wound model for fourteen days and orally in the incision wound model for ten days, respectively. Povidone iodine ointment was used as standard (5.0% w/w). Dose dependent activities resulted in both the wound models when compared to the standard (povidone iodine) and the control. Topical application of crude aqueous extract of S. rebaudiana (500 mg/kg b.w) in excision wound model decreased significantly the wound area by 15th day, i.e. 48.2±2.0 compared with control 94.1±1.2. Epithelization time was decreased from 17.3±0.21 to 12.0±0.10 and hydroxylproline content was increased from 32.2±0.11 to 67.6±0.10 when compared with control. In incision wound model breaking strength of wounds, wet and dry granulation of the tissue weight and hydroxyproline were increased significantly from control with AESR. In conclusion, AESR leaves accelerated wound healing activity in mice and thus supports its traditional use.