Human adenovirus detection among immunocompetent and immunocompromised patients presenting acute respiratory infection
Rev. Soc. Bras. Med. Trop
;
46(2): 161-165, Mar-Apr/2013. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-674638
ABSTRACT
INTRODUCTION:
Human adenoviruses (HAdV) play an important role in the etiology of severe acute lower respiratory infection, especially in immunocompromised individuals. The aim of the present study was detect the HAdV through differentmethods:
direct fluorescence assay (DFA) and nested-polymerase chain reaction (PCR-nested) from patients with acute respiratory infection (ARI) up to 7 days of symptoms onset.METHODS:
Samples (n=643) were collected from different risk groups during from 2001 to 2010 139 adults attended in an Emergency Room Patients (ERP); 205 health care workers (HCW); 69 from Renal Transplant Outpatients (RTO); 230 patients in hematopoietic stem cell transplantation (HSCT) program.RESULTS:
Among all patients (n=643) adenovirus was detected on 13.2% by DFA and/or PCR 6/139 (4.3%) adults from ERP, 7/205 (3.4%) from HCW samples, 4/69 (5.8%) from RTO and 68/230 (29.5%) from HSCT patients. Nested PCR showed higher detection (10%) compared to DFA test (3.8%) (p < 0.001). HSCT patients presented significantly higher prevalence of HAdV infection.CONCLUSIONS:
Adenovirus detection through nested-PCR assay was higher. However the inclusion of molecular method in laboratorial routine diagnostic should be evaluated considering the reality of each specific health service. .
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Infecções Respiratórias
/
Infecções por Adenovirus Humanos
Tipo de estudo:
Estudo diagnóstico
/
Estudo de prevalência
/
Fatores de risco
Limite:
Adolescente
/
Adulto
/
Idoso
/
Aged80
/
Criança
/
Feminino
/
Humanos
/
Masculino
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
Medicina Tropical
Ano de publicação:
2013
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal de São Paulo/BR
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