Model studies of crotamine self-association
Braz. j. med. biol. res
;
22(1): 17-24, 1989. ilus
Artigo
em Inglês
| LILACS
| ID: lil-67467
ABSTRACT
1. The self-association of crotamine in the concentration range from 10 to 40 microg/ml was studied at pH 6, 25-C, and at low ionic strength by monitoring the effect of protein concentration on the absorbance at 196 nm. 2. Of the several mathematical models tested, an equilibrium between monomers and trimers with an association constant of 1.50 x 10 M-2 gives an adequate representation of the phenomenon. However, a non-ideal, two-stage model describing an equilibrium among monomers, dimers, and trimers gives the best fit of the experimental data. 3. The equilibrium constants found for dimerization and trimerization were 1.70 x 10 M-1 and 3.37 x10 M-1, respectively. 4. This model was confirmed by polyacrylamide gel electrophoresis where a trimer band was separated from an interconverting monomer-dimer band
Buscar no Google
Índice:
LILACS (Américas)
Assunto principal:
Venenos de Crotalídeos
/
Eletroforese em Gel de Poliacrilamida
Tipo de estudo:
Fatores de risco
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
1989
Tipo de documento:
Artigo
/
Documento de projeto
Similares
MEDLINE
...
LILACS
LIS