Molecular basis of high-level ciprofloxacin resistance in Neisseria gonorrhoeae strains from Shandong Province, China
Braz. j. microbiol
;
44(1): 273-276, 2013.
Artigo
em Inglês
| LILACS
| ID: lil-676902
ABSTRACT
In the study, the ciprofloxacin resistance rate was 100%. High-level ciprofloxacin resistance rate was 63.55%. Sixteen different mutation patterns involved in the formation of ciprofloxacin resistance were identified. The most prevalent were patterns P7 (25.2%), P8 (15.0%), P9 (11.2%), P1 (10.3%), and P5 (10.3%). All of the 107 NG isolates analyzed for mutations in the study have demonstrated a change of Ser-91 → Phe in the gyrA gene, and all except one have demonstrated a change in position 95 of the amino acid sequence. All of the 68 high-level QRNG isolates had double mutations in gyrA gene combined with a single or two mutations in parC gene. It is most important that a new mutation site of Ile-97 → Met in gyrA and a new mutation of Leu-106 → Ile in parC were found in the study, both leading to high-level ciprofloxacin resistance (MIC values, 8 µg/mL, 32 µg/mL, respectively). Therefore, we confim that gyrA mutations are necessary for the fluoroquinolone resistance phenotype and parC mutations are correlated intimately with high-level fluoroquinolone resistance. In China fluoroquinolone resistance in Neisseria gonorrhoeae strains is very serious and the new mutation sites in the fluoroquinolone resistance-determining regions emerge more and more quickly. Hence, in China fluoroquinolones, which are used to treat gonorrhoea presently, should be substituted by a new antibiotics.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Técnicas In Vitro
/
Resistência Microbiana a Medicamentos
/
Ciprofloxacina
/
Gonorreia
/
Reação em Cadeia da Polimerase
/
Suscetibilidade a Doenças
/
Antibacterianos
/
Neisseria gonorrhoeae
Tipo de estudo:
Estudo de prevalência
/
Fatores de risco
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2013
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Taian Central Hospital/CN
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