Characterization of a virulent Leptospira interrogans strain isolated from an abandoned swimming pool
Braz. j. microbiol
;
44(1): 165-170, 2013. ilus
Artigo
em Inglês
| LILACS
| ID: lil-676904
ABSTRACT
Pathogenic Leptospira spp. are the etiological agents of leptospirosis, an important disease of both humans and animals. In urban settings, L. interrogans serovars are the predominant cause of disease in humans. The purpose of this study was to characterize a novel Leptospira isolate recovered from an abandoned swimming pool. Molecular characterization through sequencing of the rpoB gene revealed 100% identity with L. interrogans and variable-number tandem-repeat (VNTR) analysis resulted in a banding pattern identical to L. interrogans serogroup Icterohaemorrhagiae, serovar Copenhageni or Icterohaemorrhagiae. The virulence of the strain was determined in a hamster model of lethal leptospirosis. The lethal dose 50% (LD50) was calculated to be two leptospires in female hamsters and a histopathological examination of infected animals found typical lesions associated with severe leptospirosis, including renal epithelium degeneration, hepatic karyomegaly, liver-plate disarray and lymphocyte infiltration. This highly virulent strain is now available for use in further studies, especially evaluation of vaccine candidates.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Doença de Weil
/
Técnicas In Vitro
/
Sequência de Bases
/
Reação em Cadeia da Polimerase
/
Genoma Bacteriano
/
Área Urbana
/
Leptospira interrogans serovar icterohaemorrhagiae
/
Mucosa Intestinal
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2013
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal de Pelotas/BR
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