Isolation of Staphylococcus from minas frescal type cheese and detection of enterotoxin genes
Rev. Inst. Adolfo Lutz
;
71(2): 250-258, abr.-jun. 2012. tab
Artigo
em Inglês
| LILACS, SES-SP, SESSP-CTDPROD, SES-SP, SESSP-ACVSES, SESSP-IALPROD, SES-SP, SESSP-IALACERVO
| ID: lil-688220
ABSTRACT
This work aimed at isolating Staphylococcus spp. from minas frescal type cheese, and for this purpose a Multiplex PCR (M-PCR) was standardized for detecting the classical Staphylococcus aureus enterotoxingenes (sea, seb, sec, sed and see), using the femA gene as a positive control for S. aureus strains. Bacterial detection directly from the minas frescal type cheese and from artificially contaminated food was tested.One hundred eleven colonies (104 coagulase-positive and seven coagulase-negative) were selected for performing M-PCR assay. Thirty-four colonies (30.62%) were positive for at least one of the fiveente rotoxin genes analyzed; enterotoxins sea and seb were the most frequently detected. The study on Staphylococci coagulase-positive samples revealed that 40% of the samples showed bacterial counts above the limit established by Brazilian legislation.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Staphylococcus
/
Queijo
/
Reação em Cadeia da Polimerase
/
Enterotoxinas
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Rev. Inst. Adolfo Lutz
Ano de publicação:
2012
Tipo de documento:
Artigo
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