Yeast biomass production: a new approach in glucose-limited feeding strategy
Braz. j. microbiol
;
44(2): 551-558, 2013. ilus, graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-688588
ABSTRACT
The aim of this work was to implement experimentally a simple glucose-limited feeding strategy for yeast biomass production in a bubble column reactor based on a spreadsheet simulator suitable for industrial application. In biomass production process using Saccharomyces cerevisiae strains, one of the constraints is the strong tendency of these species to metabolize sugars anaerobically due to catabolite repression, leading to low values of biomass yield on substrate. The usual strategy to control this metabolic tendency is the use of a fed-batch process in which where the sugar source is fed incrementally and total sugar concentration in broth is maintained below a determined value. The simulator presented in this work was developed to control molasses feeding on the basis of a simple theoretical model in which has taken into account the nutritional growth needs of yeast cell and two input data the theoretical specific growth rate and initial cell biomass. In experimental assay, a commercial baker's yeast strain and molasses as sugar source were used. Experimental results showed an overall biomass yield on substrate of 0.33, a biomass increase of 6.4 fold and a specific growth rate of 0.165 h-1 in contrast to the predicted value of 0.180 h-1 in the second stage simulation.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Saccharomyces cerevisiae
/
Biomassa
/
Glucose
Tipo de estudo:
Estudo prognóstico
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2013
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Estadual de Campinas/BR
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