Genetic variants associated with addictive behavior in Colombianaddicted and non-addicted to heroin or cocaine / Variantes genéticas asociadas con conducta adictiva en colombianos adictos y no adictos a heroína o cocaína

ABSTRACT

Objective:Determine the prevalence and compare somegenetic markers involved in addictive behavior in a groupof addicts to derivative of coca (cocaine/crack) or heroinand a control group of non-addicted people matched forgender, age and ethnicity.Methods:A 120 addicts and 120 non-addicts Colombianmale were surveyed and genotyped for 18 polymorphismof the OPRM1, DRD2, DRD4, SLC6A3, SLC6A4, ABCB1,DβH and CYP2B6 genes. For the identification of allelesmarkers were used mini-sequencing and fragment multi-plex PCR techniques; ethnicity of cases and controls wasanalyzed with 61 AIMs.Results:The age of onset use of heroin or coca derivati-ves (cocaine/crack) was 16,5±6 years and 99,2 percent of themconsume several illicit drugs. It showed that controlsand addicts belong to the same ethnic group. Significantdifferences between addicts and controls in relation toschooling, marital status, social security family historyof substance abuse (p<0,001), Int8-VNTR SLC6A3 gene(p=0,015) and SNP 3435C>T ABCB1 gene (p=0,001) werefound.Conclusion:The present results indicate that the VNTR-6R polymorphism of the gene SLC6A3 and the genoty-pe 3435CC in the ABCB1 gene, are both associated withaddictive behavior to heroin or cocaine.