High passage numbers induce resistance to apoptosis in C2C12 muscle cells
Biocell
;
37(1): 1-9, Apr. 2013. ilus, graf
Artigo
em Inglês
| LILACS
| ID: lil-694715
ABSTRACT
Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as l-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2O2 induction of apoptosis. We propose serially cultured C2C12 cells as a refractory model to H2O2-induced apoptosis. In addition, the data obtained in this work suggest that mtDNA is required for apoptotic cell death in skeletal muscle C2C12 cells.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Oxidantes
/
Apoptose
/
Mioblastos Esqueléticos
/
Peróxido de Hidrogênio
/
Mitocôndrias
Limite:
Animais
Idioma:
Inglês
Revista:
Biocell
Assunto da revista:
Clulas
Ano de publicação:
2013
Tipo de documento:
Artigo
País de afiliação:
Argentina
Instituição/País de afiliação:
Universidad Nacional del Sur/AR
Similares
MEDLINE
...
LILACS
LIS