Poly (2'-0-methyladenylic acid) as template of reverse transcriptase
Arq. biol. tecnol
;
31(2): 313-20, 1988. tab
Artigo
em Inglês
| LILACS
| ID: lil-69596
RESUMO
Poly (2'-O-methyladenylic acid), poly (Am), is recognized as template in the reaction catalyzed by avian myeloblastosis virus, AMV, reverse transcriptase better in the presence of Mn than Mg as divalent cation. An apparent KM value of 2.5 x 10 M in relation to TTP and an inhibition of the reaction at poly (Am concentration higher than 5 microng/ml have been observed. Ethidium bromide, EtBr, tetramethyl ethidium bromide, TMEtBr, dideoxy TTP, and novobiocin are inhibitors of the poly (Am)-directed reverse transcriptase reaction in the presence of Mn
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Índice:
LILACS (Américas)
Assunto principal:
Poli A
/
Moldes Genéticos
/
DNA Polimerase Dirigida por RNA
Idioma:
Inglês
Revista:
Arq. biol. tecnol
Assunto da revista:
Biologia
/
Biotecnologia
Ano de publicação:
1988
Tipo de documento:
Artigo
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