Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
Mem. Inst. Oswaldo Cruz
;
108(7): 832-835, 1jan. 2013. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-696013
ABSTRACT
Prolonged culturing of many microorganisms leads to the loss of virulence and a reduction of their infective capacity. However, little is known about the changes in the pathogenic strains of Acanthamoeba after long culture periods. Our study evaluated the effect of prolonged culturing on the invasiveness of different isolates of Acanthamoeba in an in vivo rat model. ATCC strains of Acanthamoeba, isolates from the environment and clinical cases were evaluated. The in vivo model was effective in establishing the infection and differentiating the pathogenicity of the isolates and re-isolates. The amoebae cultured in the laboratory for long periods were less virulent than those that were recently isolated, confirming the importance of passing Acanthamoeba strains in animal models.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Virulência
/
Acanthamoeba
/
Cultura Axênica
/
Amebíase
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2013
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal do Rio Grande do Sul/BR
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