COI barcode versus morphological identification of Culex ( Culex ) (Diptera: Culicidae) species: a case study using samples from Argentina and Brazil
Mem. Inst. Oswaldo Cruz
;
108(supl.1): 110-122, 2013. tab, graf
Artigo
em Inglês
| LILACS
| ID: lil-697832
ABSTRACT
Sequences of the cytochrome c oxidase subunit I (COI) mitochondrial gene from adults of 22 Culex ( Culex ) species from Argentina and Brazil were employed to assess species identification and to test the usefulness of COI for barcoding using the best close match (BCM) algorithm. A pairwise Kimura two-parameter distance matrix including the mean intra and interspecific distances for 71 COI barcode sequences was constructed. Of the 12 COI lineages recovered in the Neighbour-joining topology, five confirmed recognised morphological species ( Cx. acharistus , Cx. chidesteri , Cx. dolosus , Cx. lygrus and Cx. saltanensis ) with intraspecific divergences lower than 1.75%. Cx. bilineatus is formally resurrected from the synonymy of Cx. dolosus . Cx. maxi , Cx. surinamensis and the Coronator group species included were clustered into an unresolved lineage. The intraspecific distance of Cx. pipiens (3%) was almost twice the interspecific between it and Cx. quinquefasciatus (1.6%). Regarding the BCM criteria, the COI barcode successfully identified 69% of all species. The rest of the sequences, approximately 10%, 18% and 3%, remained as ambiguously, mis and unidentified, respectively. The COI barcode does not contain enough information to distinguish Culex ( Cux. ) species.
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Assunto principal:
Classificação
/
Complexo IV da Cadeia de Transporte de Elétrons
/
Culex
/
Código de Barras de DNA Taxonômico
/
Sistemas de Identificação Animal
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
País/Região como assunto:
América do Sul
/
Argentina
/
Brasil
Idioma:
Inglês
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
Medicina Tropical
/
Parasitologia
Ano de publicação:
2013
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Argentina
Instituição/País de afiliação:
Universidad Nacional de Cordoba/AR
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