Use of Plackett-Burman design for rapid screening of nitrogen and carbon sources for the production of lipase in solid state fermentation by Yarrowia lipolytica from mustard oil cake (Brassica napus)
Braz. j. microbiol
;
44(3): 915-921, July-Sept. 2013. graf, tab
Artigo
em Inglês
| LILACS
| ID: lil-699781
ABSTRACT
Mustard oil cake (Brassica napus), the residue obtained after extraction of mustard oil from mustard oil seeds, was investigated for the production of lipase under solid state fermentation (SSF) using the marine yeast Yarrowia lipolytica NCIM 3589. Process parameters such as incubation time, biomass concentration, initial moisture content, carbon source concentration and nitrogen source concentration of the medium were optimized. Screening of ten nitrogen and five carbon sources has been accomplished with the help of Plackett-Burman design. The highest lipase activity of 57.89 units per gram of dry fermented substrate (U/gds) was observed with the substrate of mustard oil cake in four days of fermentation.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Carbono
/
Yarrowia
/
Brassica napus
/
Lipase
/
Nitrogênio
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2013
Tipo de documento:
Artigo
País de afiliação:
Índia
Instituição/País de afiliação:
GITAM University/IN
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