A roadmap to directed enzyme evolution and screening systems for biotechnological applications
Biol. Res
;
46(4): 395-405, 2013. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-700402
ABSTRACT
Enzymes have been long used in man-made biochemical processes, from brewing and fermentation to current industrial production of fine chemicals. The ever-growing demand for enzymes in increasingly specific applications requires tailoring naturally occurring enzymes to the non-natural conditions found in industrial processes. Relationships between enzyme sequence, structure and activity are far from understood, thus hindering the capacity to design tailored biocatalysts. In the field of protein engineering, directed enzyme evolution is a powerful algorithm to generate and identify novel and improved enzymes through iterative rounds of mutagenesis and screening applying a specific evolutive pressure. In practice, critical checkpoints in directed evolution are selection of the starting point, generation of the mutant library, development of the screening assay and analysis of the output of the screening campaign. Each step in directed evolution can be performed using conceptually and technically different approaches, all having inherent advantages and challenges. In this article, we present and discuss in a general overview, challenges of designing and performing a directed enzyme evolution campaign, current advances in methods, as well as highlighting some examples of its applications in industrially relevant enzymes.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Biotecnologia
/
Engenharia de Proteínas
/
Evolução Molecular Direcionada
/
Enzimas
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2013
Tipo de documento:
Artigo
País de afiliação:
Alemanha
Instituição/País de afiliação:
RWTH Aachen University/DE
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