Recovering Streptococcus mutans over restorations by the tray technique: a randomized clinical study
Braz. j. oral sci
;
12(4): 292-297, Oct.-Dec. 2013. ilus, tab
Artigo
em Inglês
| LILACS
| ID: lil-701316
ABSTRACT
AIM:
To correlate the tray technique for isolation and counting of Streptococcus mutans over dental restoration with the toothpick technique.METHODS:
Forty 18-50-year-old patients of both genders with high cariogenic risk were selected. Inclusion criteria were occlusal restoration evaluated as alpha, according to the Ryge's criteria (examiner' Cohen-Kappa 0.8) and 2 years old maximum age. Each patient provided a saliva sample (control group S) and two samples of dental biofilm from amalgam or resin-based composite restorations in the same patient, one obtained with the toothpick technique (group TT), and the other from homologous tooth restoration with the tray technique (group TR). This method involves only a direct impression on the occlusal surfaces by a section of a fluoride tray with trypticase yeast extract cysteine sucrose with bacitracin (TYCSB) agar previously prepared and solidified. The samples were incubated at 37 ºC for 48 h and the colony-forming units (CFU) were counted. The results were statistically analyzed with SPSS software using the linear regression method at 95% confidence level.RESULTS:
TR was able to isolate S. mutans, and there was a significant positive correlation (r=0.95) with TT. There was no significant correlation between TR and control group S (r=0.47).CONCLUSIONS:
TR was able to isolate and count S. mutans from the dental biofilm of dental restorations with a significant correlation with the TT group.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Streptococcus mutans
/
Modelos Lineares
/
Cárie Dentária
Tipo de estudo:
Ensaio Clínico Controlado
/
Estudo prognóstico
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Braz. j. oral sci
Assunto da revista:
Odontologia
Ano de publicação:
2013
Tipo de documento:
Artigo
País de afiliação:
Brasil
/
Chile
Instituição/País de afiliação:
University Estadual Paulista/BR
/
University of Chile/CL
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